Journal: Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Article Title: Unleashing the Power of Cold Atmospheric Plasma: Inducing Mitochondria Damage-Mediated Mitotic Catastrophe.

doi: 10.1002/advs.202401842

Figure Lengend Snippet: Figure 4. CAP treatment suppressed microtubule dynamics and centrosome maturation. A) Representative immunofluorescent images of 𝛼-tubulin in Calu-1 cells at 24 h after CAP treatment. Scale bar, 10 μm. B) Representative immunofluorescent images of 𝛼-tubulin network in Calu-1 cells at 24 h after CAP treatment with or without Nocodazole (5 μM) treatment. Scale bar, 100 μm. C) Representative immunofluorescent images of Aurora A in Calu-1 cells at 24 h after CAP treatment. Scale bar, 10 μm. D) Frequency of mitotic cells with bipolar spindles. E) The expression of related proteins in Calu-1 and H1299 at 24 h after CAP treatment.

Article Snippet: The primary antibodies against AMPK (1:1000, 5831S), phosphoAMPKα (Thr172) (1:1000, 50081S), acly-α-tubulin (1:1000, 5335), Aurora A (1:1000, 14475S), phospho-Aurora A (Thr288) (1:1000, 3079S) from Cell Signaling Technology, α-tubulin from Proteintech (1:1000, 11224-1-AP) and β-actin from HuaAn biotechnology (1:2000, EM21002) were used.

Techniques: Expressing

Journal: Cell death discovery

Article Title: Selective molecular inhibition of the HDAC6 ZnF-UBP binding domain impairs multiple myeloma cell function.

doi: 10.1038/s41420-025-02465-1

Figure Lengend Snippet: Fig. 4 The molecular inhibition of the HDAC6 ZnF-UBP binding domain did not impair the HDAC6 expression or its catalytic activity in RPMI 8226 cells but impaired the aggresome formation. A Expression of HDAC6 and acetylated tubulin (Ac-tub (K40)) in wild-type, HDAC6KO

Article Snippet: The following primary antibodies were used: HDAC6 (Cell Signaling Technology, Danvers, MA, USA, #7558, 1:500), acetylated tubulin (Cell Signaling Technology #3971, 1:1000), GADPH (Merck, Burlington, MA, USA, MAB374, 1:500).

Techniques: Inhibition, Binding Assay, Expressing, Activity Assay